The use of CRISPR-based genome editing has increased significantly since its discovery in bacteria. And the number of applications for the technology — from research to drug development and therapies, plant breeding, diagnostics, and more — seem endless, especially given the development of more versatile and flexible nucleases, or more precise methods such as base editing and prime editing.
But off-target effects continue to be a challenge to overcome, as has capturing the full scope of those effects, from their impact on DNA to how they affect the transcriptome and beyond. In this roundtable discussion, a panel of gene-editing experts discussed the methods that are being developed to find, assess, and minimize the effects of off-target edits that result from CRISPR-based genome editing and base editing. Our panel will covered a range of topics, including reliable methods for quality control of those effects, what these off-target detection methods have revealed about genome editing, and how they could help CRISPR researchers perfect the technology and broaden its applicability.
Dr. Kiana Aran Featured Speaker on Genome Web Panel – Emerging Methods to Minimize Off-Target Effects of Genome Editing: A Stakeholder Panel Discussion
September 17, 2021